Antibody（cell proliferation, mitosis）｜一般社団法人　日本細胞生物学会

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Antibody（cell proliferation, mitosis）

Antibody（cell proliferation, mitosis）

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	4%PFA/PBS
	rabbit

	mouse oocyte
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	mouse oocyte
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	4%PFA/PBS
	rabbit

	HeLa
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	HeLa
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	MEF
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	NIH3T3
phospho-AuroraA/AIK (T288)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3091	ice cold Methanol
	rabbit

	HeLa
phospho-AuroraA/AIK (T288)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3091	ice cold Methanol
	rabbit

	MEF
phospho-AuroraA/AIK (T288)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3091	ice cold Methanol
	rabbit

	NIH3T3
AuroraA/AIK2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3092	ice cold Methanol
	rabbit

	HeLa
AuroraA/AIK2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3092	ice cold Methanol
	rabbit

	MEF
AuroraA/AIK2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3092	ice cold Methanol
	rabbit

	NIH3T3
AuroraB2011-09-27	EP1009Y	WB	IF	IHC	IP
	EPT		100
	1788-1
	rabbit
	m	TCA, 4%PFA, 1%PFA, MeOH
	HeLa
phospho-AuroraB (T232)2014-07-11		WB	IF	IHC	IP
	ROCKLAND		x400
	600-401-677	4%PFA/PBS
	rabbit

	HeLa
AuroraB/AIM-12014-06-04		WB	IF	IHC	IP
	BD Transduction laboratories	x500	x500
	611082	3%PFA (in PBS)
	mouse
	IgG
	HeLa	Kiyomitsu et al., JCB 2010
AKAP4502018-08-09	Clone 7	WB	IF	IHC	IP
	BD Biosciences		1/500
	611518	MeOH
	Mouse

	RPE1, HeLa, U2OS
ASPM2018-07-12		WB	IF	IHC	IP
	Novus Biologicals		1/250
	NB100-2278	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
APC2011-08-10	Ab-1 FE9	WB	IF	IHC	IP
	Calbiochem	x1000
	OP44
	mouse
	m

APC2011-08-10	Ab-5 CF11	WB	IF	IHC	IP
	Calbiochem	x1000
	OP62
	mouse
	m

APC2011-08-10	Ab-7 CC-1	WB	IF	IHC	IP
	Calbiochem	x1000
	OP80
	mouse
	m

α-tubulin2018-07-12		WB	IF	IHC	IP
	MBL		1/500
	PM054	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
human APC2011-08-10	C-20	WB	IF	IHC	IP
	Santa Cruz	x1000
	sc896
	rabbit
	p

human APC2011-08-10	F-3	WB	IF	IHC	IP
	Santa Cruz	x1000
	sc9998
	mouse
	m

α-tubulin2018-07-12		WB	IF	IHC	IP
	Sigma		1/1000
	T5168	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【B】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
BrdU (& IdU but weak CldU)2012-12-11		WB	IF	IHC	IP
	BD pharmingen			x100
	347580	4%PFA (in PBS)
	mouse	HCl, RT, 30m

	mouse brain
bromodeoxyuridine2011-08-10	BrdU	WB	IF	IHC	IP
	BD Bioscience		x100
	555627	3%PFA (in PBS)
	mouse
		特殊な前処理が必要（普通に染めたら染まらないので注意） Pretreatment should be required unless no signal.
	マウス小腸
Bub32014-06-04		WB	IF	IHC	IP
	BD Transduction laboratories	x500
	611731
	mouse
	IgG
	HeLa	Kiyomitsu et al., Dev Cell 2007
BrdU (&CldU but not IdU)2012-12-11		WB	IF	IHC	IP
	abcam			x100
	ab6320	4%PFA (in PBS)
	rat	HCl, RT, 30m

	mouse brain
BrdU2011-10-13	OS94.6	WB	IF	IHC	IP
	oncogene		x100~
	NA20
	mouse	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	Mono, IgG1,k
	HeLa細胞
BrdU2011-10-13	BC1/75(ICR1)	WB	IF	IHC	IP
	Oxford Biotechnology		x100~
	OBT0030S
	rat	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	Mono, IgG2a
	HeLa細胞

【C】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Cyclin A2012-12-11		WB	IF	IHC	IP
	Santa Cruz			x100
		4%PFA (in PBS)
	rabbit	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG
	mouse brain
mouse/human CD442011-08-10	clone:IM7	WB	IF	IHC	IP
	BioLegend
	103001
	rat


CPAP/CENP-J2018-07-12		WB	IF	IHC	IP
	Proteintech		1/100
	11517-1-AP	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
CP1102018-07-12		WB	IF	IHC	IP
	proteintech		1/1000
	12780-1-AP	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
human c-kit2011-08-10	K963	WB	IF	IHC	IP
	生化学工業 Seikagaku coporation		x100
	260020	3%PFA (in PBS)
	rabbit
	p
	マウス精巣Sertoli細胞
CENPF/mitosin2018-08-09	Clone 11	WB	IF	IHC	IP
	BD Biosciences		1/100
	610768	MeOH
	Mouse

	RPE1, HeLa, U2OS
Cep1922018-07-12		WB	IF	IHC	IP
	Bethyl laboratories		1/1000
	A302-324A	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Cep1522018-07-12		WB	IF	IHC	IP
	Bethyl laboratories		1/1000
	A302-480A	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
CEP1282018-07-26		WB	IF	IHC	IP
	abcam		1/500
	ab118797	MeOH
	Rabbit

	RPE1, HeLa, U2OS
murine CD44var2011-08-10	v6	WB	IF	IHC	IP
	Bender MedS		x100
	BMS145	3%PFA (in PBS)
	rat
	m
	マウス小腸
CDK5RAP22018-07-12		WB	IF	IHC	IP
	Bethyl laboratories		1/500
	IHC00063	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
cyclinD32011-10-13	DCS-22	WB	IF	IHC	IP
	LAB VISION	X200
	MS215-P0
	mouse
	Mono, IgG1/k
	HeLa細胞
cyclin E2011-10-13		WB	IF	IHC	IP
	LAB VISION	X200
	RB-012-P0
	rabbit
	Poly
	HeLa細胞
cyclin A2011-10-13	Ab-7	WB	IF	IHC	IP
	LAB VISION	x2000
	RB-1548-P0
	rabbit
	Poly
	HeLa細胞
cdc2 P342011-10-13		WB	IF	IHC	IP
	Santa Cruz		x100
	sc-54
	mouse	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
	Mono, IgG2a
	HeLa細胞
cyclinB12011-10-13	H-433	WB	IF	IHC	IP
	Santa Cruz		x200
	sc-752
	rabbit	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
	Poly
	HeLa細胞
human c-Fos2011-08-10	4	WB	IF	IHC	IP
	Santa Cruz
	sc52
	rabbit


human c-Fos2011-08-10	4	WB	IF	IHC	IP
	Santa Cruz
	sc52
	rabbit


human c-Myc2011-08-10	N-262	WB	IF	IHC	IP
	Santa Cruz		x100
	sc764	3%PFA (in PBS)
	rabbit
	p
	マウス小腸
human c-Myc2011-08-10	C-19	WB	IF	IHC	IP
	Santa Cruz		x100
	sc788	3%PFA (in PBS)
	rabbit
	p
	マウス小腸

【D】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Dacapo2014-10-06	NP1	WB	IF	IHC	IP
	DHSB		x6
		4%PFA (in phosphate buffer)
	mouse
	IgG2b
	Drosohila imaginal disc

【E】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
p44/42 MAPK (ERK1/2)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech	x1000	x400
	#4695	4%PFA/PBS
	rabbit

	mouse oocyte
p44/42 MAPK (ERK1/2)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#4695	ice cold Methanol
	rabbit

	mouse oocyte
phopho-p44/42 MAPK (ERK1/2) (T202/Y204)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech	x1000	x400
	#9101	4%PFA/PBS
	rabbit
		signal decreases from 3h after oocyte activation
	mouse oocyte
phopho-p44/42 MAPK (ERK1/2) (T202/Y204)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#9101	ice cold Methanol
	rabbit
		signal decreases from 3h after oocyte activation
	mouse oocyte
EGFR2014-10-06	C-273	WB	IF	IHC	IP
	Abcam		x100
	ab49966	4%PFA (in phosphate buffer)
	mouse
	IgG1
	Drosohila imaginal disc
mouse EphB32011-08-10		WB	IF	IHC	IP
	R&D Systems		x100
	AF432	3%PFA (in PBS)
	goat

	マウス小腸
mouse Ephrin-B12011-08-10		WB	IF	IHC	IP
	R&D Systems		x100
	AF473	3%PFA (in PBS)
	goat

	マウス小腸

【F】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
FosB2011-08-10	102	WB	IF	IHC	IP
	Santa Cruz
	sc48
	rabbit
	p

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GM1302018-08-09		WB	IF	IHC	IP
	Cell Signaling	1/3000	1/5000
	12480	MeOH, PFA
	Rabbit	0.05% triton X-100 for PFA fixation

	RPE1, HeLa, U2OS
GPR492011-08-14		WB	IF	IHC	IP
	EPITOMICS
	2495-S	3%PFA (in PBS), methanol
	rabbit
	m
	マウス小腸
γ-tubulin2018-07-26	TU-30	WB	IF	IHC	IP
	abcam		1/500
	ab27074	MeOH
	Mouse

	RPE1, HeLa, U2OS
GPR492011-08-10 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam		x100
	ab75850	3%PFA (in PBS), methanol
	rabbit
	IgG
	マウス小腸

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
γ-tubulin2014-06-04	H-183	WB	IF	IHC	IP
	SANTA CRUZ		x200		x400
	sc-10732	MeOH
	rabbit
	IgG
	HeLa・mouse oocyte （IF only）

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Galpha-i2014-06-04		WB	IF	IHC	IP
	Santa Cruz Biotechnology		x20
	sc-56536	4%FA in PHEM
	mouse
	IgG	Use TBS instead PBS as buffer.
	HeLa	Kiyomitsu and Cheeseman, NCB 2012
human GADD1532011-08-10	R-20	WB	IF	IHC	IP
	Santa Cruz		x100
	sc793	3%PFA (in PBS)
	rabbit
	p
	マウス小腸
GPR492011-08-10	Acris	WB	IF	IHC	IP
	Acris Antibodies		x100
	SP4233P	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
γ-tubulin2014-06-04	DQ-19	WB	IF	IHC	IP
	SIGMA	x1000			x400
	T3195
	rabibt
	IgG
	HeLa

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
γ-tubulin2018-07-12	GTU88	WB	IF	IHC	IP
	Sigma		1/1000
	T6557	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【H】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human HB-EGF2011-10-12	H6	WB	IF	IHC	IP
	Santa Cruz	1	1	抗血清1:50
		【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定 Fix the freshly frozen sections with cold acetone
	rabbit
	(polyclonal)	【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】G54-R73/pro-domain
	【IF】HB-EGF強制発現Vero細胞【IHC】種々のヒト生検組織	Mol.Biol.Cell1995;6:967-980；J Histochem Cytochem 2001;49:439-444
human HB-EGF2011-10-12	M6	WB	IF	IHC	IP
	Santa Cruz

	rabbit
	(polyclonal)	【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E195-H208/CTF
		mekada, unpublished
human HB-EGF2011-10-12	3H4	WB	IF	IHC	IP
	Bio Academia	1	0.1		0.1
		【IF】4%PFA
	mouse
	Mono,IgG1	【WB】非還元のみ反応【増殖因子活性阻害】(-)【シェディング阻害】(+++)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
	【IF】HB-EGF強制発現Vero細胞	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	1F3	WB	IF	IHC	IP
	Bio Academia	1	0.1		2
		【IF】4%PFA
	mouse
	Mono,IgG1	【WB】非還元のみ反応【増殖因子活性阻害】(-)【Dt結合阻害】(+++)：３ug/ml【シェディング阻害】(+++)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
	【IF】HB-EGF強制発現Vero細胞	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	5D9	WB	IF	IHC	IP
	Bio Academia		1	10	0.1
		【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定 Fix the freshly frozen sections with cold acetone
	mouse
	Mono,IgG2a	【増殖因子活性阻害】(-)【Dt結合阻害】(+++)：３ug/ml【シェディング阻害】(++)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
	【IF】HB-EGF強制発現Vero細胞【IHC】ヒト子宮頸癌生検組織	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	7E10	WB	IF	IHC	IP
	Bio Academia		0.1		2
		【IF】4%PFA
	mouse
	Mono,IgG1	【増殖因子活性阻害】(-)【Dt結合阻害】(+++)：３ug/ml【シェディング阻害】(++)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】I133/H135/EGF-domain
	【IF】HB-EGF強制発現Vero細胞	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	3D9	WB	IF	IHC	IP
	Bio Academia		0.1		2
		【IF】4%PFA
	mouse
	Mono,IgG1	【増殖因子活性阻害】(-) 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】I133/H135/EGF-domain
	【IF】HB-EGF強制発現Vero細胞	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	6C11	WB	IF	IHC	IP
	Bio Academia		1	10	2
		【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定 Fix the freshly frozen sections with cold acetone
	mouse
	Mono,IgG2a	【増殖因子活性阻害】(-)【シェディング阻害】(+)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】N-upstream of EGF-domain
	【IF】HB-EGF強制発現Vero細胞【IHC】ヒト子宮頸癌生検組織	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	KM3566	WB	IF	IHC	IP
	Kyowa Hakko Kirin	1	1		1
		【IF】4%PFA
	mouse
	Mono,IgG1	【増殖因子活性阻害】(+++)：３０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】EGF-domain
	【IF】HB-EGF強制発現Vero細胞	Clin.CancerRes.in press
monkey CD92011-10-12	7	WB	IF	IHC	IP
		1	1	抗血清1:100	1

	mouse
	Mono,IgG2a	【WB】非還元のみ反応【Dt結合阻害】(++)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】2nd loop
	【IF】Vero細胞など【IHC】種々のヒト生検組織	J.Biol.Chem.1991;266:20463-20469；J Histochem Cytochem 2001;49:439-444
HP1alpha2014-06-04		WB	IF	IHC	IP
	Millipore	x500

	mouse
	IgG
	HeLa	Kiyomitsu et al., JCB 2010
HP1gamma2014-06-04		WB	IF	IHC	IP
	abcam	x500

	rabbit
	IgG
	HeLa	Kiyomitsu et al., JCB 2010
phospho Histone H32011-08-10	Ser10	WB	IF	IHC	IP
	Upstate		x100
	06-570	3%PFA (in PBS)
	rabbit
	p
	マウス胎児脳
phospho-Histone H3(ser10)2011-10-13		WB	IF	IHC	IP
	upstate	X2000~
	06-570
	rabbit	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	Poly
	HeLa細胞
human HB-EGF2011-10-12	4G10	WB	IF	IHC	IP
	Bio Academia	1	0.1		0.1
	71-501	【IF】4%PFA
	mouse
	Mono,IgG1	【WB】非還元のみ反応【増殖因子活性阻害】(-)【Dt結合阻害】(+++)：３ug/ml【シェディング阻害】(+++)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
	【IF】HB-EGF強制発現Vero細胞	J.Biochem.2010;148:55-69
HEC12014-06-04		WB	IF	IHC	IP
	abcam		x1000
	ab3613	3%PFA (in PBS)
	mouse
	IgG
	HeLa	Kiyomitsu et al., Dev Cell 2007
human HB-EGF2011-10-12	Anti-human HB-EGF Neutralizing Antibody	WB	IF	IHC	IP
	R&D	0.25	1
	AF-259-NA	【IF】4%PFA
	goat
	(polyclonal)	【増殖因子活性阻害】(+++)：１ug/ml【シェディング阻害】(-) 【種特異性 species specificity】human(+++)/mouse(+)【エピトープ epitope】ectodomain
	【IF】HB-EGF強制発現Vero細胞
HKLP２2011-09-27		WB	IF	IHC	IP
	Cytoskeleton		100
	AKIN13
	rabbit
	p	TCA>MeOH>1%PFA
	HeLa
human HB-EGF2011-10-12	MAB259	WB	IF	IHC	IP
	R&D	1	1	10	0.1
	MAB259	【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定 Fix the freshly frozen sections with cold acetone
	mouse
	Mono,IgG2a	【WB】非還元のみ反応【増殖因子活性阻害】(+)：１０ug/ml【Dt結合阻害】(+)：３０ug/ml【シェディング阻害】(+)：１０ug/ml 【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】H135/EGF-domain
	【IF】HB-EGF強制発現Vero細胞【IHC】ヒト子宮頸癌生検組織	J.Biochem.2010;148:55-69
human HB-EGF2011-10-12	C18	WB	IF	IHC	IP
	Santa Cruz	0.2
	sc-1413
	goat
	(polyclonal)	【種特異性 species specificity】human(+++)/mouse(+)【エピトープ epitope】CTF

mouse HB-EGF2011-10-12	M18	WB	IF	IHC	IP
	Santa Cruz	0.2
	sc-1414
	goat
	(polyclonal)	【種特異性 species specificity】human(+)/mouse(+++)【エピトープ epitope】CTF

【K】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Ki672012-12-11		WB	IF	IHC	IP
	Novocrasta			x200
	NCL-Ki67-MM1	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
		proliferation
	mouse brain
human Ki672011-08-10		WB	IF	IHC	IP
	Novocastra		x100
	NCL-Ki67p	3%PFA (in PBS)
	rabbit
	p
	マウス小腸
Ki672012-12-11		WB	IF	IHC	IP
	Novocrasta			x200
	NCL-Ki67P	4%PFA (in PBS)
	rabbit	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG	proliferation
	mouse brain
Katanin p60 AL12018-07-26	A-10	WB	IF	IHC	IP
	Santa Cruz Biotechnology		1/100
	sc-373814	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【L】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
LGN2014-06-04		WB	IF	IHC	IP
	Bethyl Laboratories	x1000
	A303-032A
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, Cell 2013

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Mad22014-06-04		WB	IF	IHC	IP
	Covance		x500
		3%PFA (in PBS)
	rabbit
	IgG	Make aliquots and store at -20℃. Storing at 4℃ sometimes inactivates this antibody.
	HeLa	Goshima et al., JCB 2003
Myc2011-08-10		WB	IF	IHC	IP
	Upstate
	6340
	rabbit


Phospho-p44/42 MAP kinase (pT202/pY204)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	9106S
	mouse
	monoclonal	4%PFA
	MDCK II
MKLP12011-09-27		WB	IF	IHC	IP
	Cytoskeleton		100
	AKIN06
	rabbit
	p	TCA, 4%PFA, 1%PFA, MeOH
	HeLa

【m】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
mAb4142014-08-01		WB	IF	IHC	IP
	COVANCE		x400
	MMS-120P	4%PFA/PBS
	mouse
	IgG1
	mouse oocyte (pronucleus)
mAb4142014-08-01		WB	IF	IHC	IP
	COVANCE		x400
	MMS-120P	ice cold Methanol
	mouse
	IgG1
	mouse oocyte (pronucleus)

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
C-Myc2011-10-13		WB	IF	IHC	IP
	Santa Cruz		X300
	sc-40
	mouse	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	Mono, IgG1
	HeLa細胞
MKLP12011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-867
	rabbit
	p	TCA, 4%PFA, 1%PFA, MeOH
	HeLa

【N】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Nup982014-08-01		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2598	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup982014-08-01		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2598	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
Nup1332014-07-11		WB	IF	IHC	IP
	Proteintech Group		x400
	12405-1-AP	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup1332014-08-01		WB	IF	IHC	IP
	Proteintech Group		x400
	12405-1-AP	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
NuMA2014-06-04		WB	IF	IHC	IP
	cell signaling		x100
	3888	3% PFA (in PBS), 4%FA in PHEM
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, NCB 2012
NuMA2018-07-26		WB	IF	IHC	IP
	Cell Signaling Technology		1/1000
	3888S	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Nup622014-08-01		WB	IF	IHC	IP
	BD transduction		x400
	610497	4%PFA/PBS
	mouse
	IgG2b
	mouse oocyte (pronucleus)
Nup622014-08-01		WB	IF	IHC	IP
	BD transduction		x400
	610497	ice cold Methanol
	mouse
	IgG2b
	mouse oocyte (pronucleus)
NuMA2014-06-04		WB	IF	IHC	IP
	abcam		x1000
	ab36999	3% PFA (in PBS), 4%FA in PHEM
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, NCB 2012
NEDD12018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab57336	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
Nup352014-08-01		WB	IF	IHC	IP
	SIGMA		x400
	HPA018401	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup352014-08-01		WB	IF	IHC	IP
	SIGMA		x400
	HPA018401	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
NDC802018-07-26	C-11	WB	IF	IHC	IP
	Santa Cruz Biotechnology		1/50
	sc-515550	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【NU】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
4.1R/EPB412014-06-04		WB	IF	IHC	IP
	Protein Tech Group	x1000
	13014-1-AP
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, Cell 2013
4.1G/EPB41L2014-06-04		WB	IF	IHC	IP
	Bethyl Laboratories	x500	x500
	A301-424A	3%PFA (in PBS), 4%FA in PHEM
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, Cell 2013

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
p272012-12-12		WB	IF	IHC	IP
	BD pharmingen			x100
		4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
		G1
	mouse brain

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
phospho-ERK(Thr202/Tyr204)2014-10-06	197G2	WB	IF	IHC	IP
	Cell Signaling		x100
	4377	4%PFA (in phosphate buffer)
	rabbit
		Use 'Can Get Signal'(TOYOBO) for staining
	Drosohila imaginal disc
phospho-Src(Y418)2014-10-06		WB	IF	IHC	IP
	Invitrogen		x100
	44660G	4%PFA (in phosphate buffer)
	rabbit

	Drosohila imaginal disc
phospho-JNK(Thr183/Tyr185) 2014-09-04	G9	WB	IF	IHC	IP
	Cell Signaling		x100
	9255	4%PFA (in phosphate buffer)
	mouse
	IgG1	Use 'Can Get Signal'(TOYOBO) for staining
	Drosohila imaginal disc
phospho-Histone H3(S10)2014-09-04		WB	IF	IHC	IP
	Cell Signaling		x100
	9701	4%PFA (in phosphate buffer)
	rabbit

	Drosohila imaginal disc
phospho-Histone H3 (Ser10)2017-10-10		WB	IF	IHC	IP
	Cell Signaing		1/100
	9701	4% PFA at 4oC over night
	Rabbit	0.5% Triton in PBS

	zebrafish larvae (1 dpf/ day post fertilization)

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
pH32012-12-12		WB	IF	IHC	IP
	Cell Signaling			x400
	9706	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
		mitosis
	mouse brain
Plk12018-07-26		WB	IF	IHC	IP
	Bethyl laboratories		1/1000
	A300?251A	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
pericentrin2018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab28144	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
Pericentrin2018-07-26		WB	IF	IHC	IP
	abcam		1/2000
	ab4448	MeOH, PFA
	Rabbit	0.05% triton X-100 for PFA fixation

	RPE1, HeLa, U2OS
pericentrin2018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab4448	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Polyglutamylation Modification2018-07-26	GT335	WB	IF	IHC	IP
	AdipoGen		1/1000
	AG-20B-0020-C100	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
Polyglutamylated tubulin2018-08-09	GT335	WB	IF	IHC	IP
	AdipoGen		1/500
	AG-20B-0020-C100	MeOH
	Mouse

	RPE1, HeLa, U2OS
mouseαPCNA2011-08-10		WB	IF	IHC	IP
	DakoCytomation		x100
	M0879	3%PFA (in PBS)
	mouse
	m
	マウス胎児脳
Plk42018-07-26	6H5	WB	IF	IHC	IP
	Merck Millipore		1/500
	MABC544	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
PCNA2011-10-13	5A10	WB	IF	IHC	IP
	MBL	X10000
	MH-12-3
	mouse
	Mono, IgG1
	HeLa細胞
p532011-10-13	DO-1	WB	IF	IHC	IP
	Thermo	X 100
	MS-187-PO
	mouse
	Mono, IgG2a
	TIG-1細胞
PCNA2012-12-12		WB	IF	IHC	IP
	Oncogene research			x200
	NA03	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min

	mouse brain
human PCNA2011-08-10	FL-261	WB	IF	IHC	IP
	Santa Cruz
	sc7907
	rabbit
	p

【R】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
RhoA/C2011-09-27	Lulu51	WB	IF	IHC	IP
	Yonemura		50-100

	rat
	m
	HeLa, MDCK II, EpH4, 3Y1, P3, L, ほとんどのマウス組織
RhoA2011-09-27	おそらく26C4と同クローン (probably clone 26C4)	WB	IF	IHC	IP
	ARP		100
	01-4301M
	mouse
	m	TCA おそらく26C4ど同クローン
	HeLa
RhoABC2011-09-27	clone 55	WB	IF	IHC	IP
	Upstate		100
	05-778
	mouse
	m	TCA
	HeLa
Rho (N-term)2011-09-27		WB	IF	IHC	IP
	EPT		100
	1600-1
	rabbit
	m	TCA 染色は弱い Weak staining
	HeLa
Rho2011-09-27	clone EP487Y	WB	IF	IHC	IP
	EPT		100
	1667-1
	rabbit
	m	分裂溝はTCAのみだが弱い Cleavage furrows can be weakly stained only by TCA fix.
	HeLa
RhoA (67B9)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	2117
	rabbit
	m	TCA
	HeLa
Ras2014-10-06		WB	IF	IHC	IP
	Cell Signaling		x100
	3965	4%PFA (in phosphate buffer)
	rabbit

	Drosohila imaginal disc
RhoA2011-09-27	おそらく26C4と同クローン (probably clone 26C4)	WB	IF	IHC	IP
	Cytoskeleton		100
	ARH01
	m
	m	TCAのみOK Visualized only by TCA fix.
	HeLa，MDCK II
RACGAP12011-09-27	clone 1G6	WB	IF	IHC	IP
	Abnova		100
	H00029127-M01
	m
	m	MeOHでmidobodyのみわかる程度で弱い Only midbody can be weakly visualized by MeOH fix.
	HeLa
RACGAP1/MgcRacGAP2011-09-27		WB	IF	IHC	IP
	IMGENEX		100
	IMG-3464
	goat
	p	弱い（midbodyのみ） Only midbody can be weakly visualized.
	HeLa
Rho2011-09-27		WB	IF	IHC	IP
	BD		100
	R73920
	m
	m
	HeLa
RhoA/B/C2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-179
	rabbit
	p	TCAのみOK Visualized only by TCA fix.
	HeLa, MECK II, ほとんどのマウス、ラット組織
RhoA2011-09-27	26C4	WB	IF	IHC	IP
	Santa Cruz		100
	SC418
	m
	m	TCAのみOK Visualized only by TCA fix.
	HeLa, MDCK II, EpH4, 3Y1, P3, L, ほとんどのラット組織

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Src2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-8056
	mouse
	m	Focal adhesion染まらず。おそらく使用不可 Not stain focal adhesion.
	HeLa

【w】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Wingless2014-09-04	4D4	WB	IF	IHC	IP
	DHSB		x100
		4%PFA (in phosphate buffer)
	mouse
	IgG1
	Drosohila imaginal disc